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Total RNA Extraction, cDNA Synthesis and Real Time PCR. Homogenize the tissue in the nucleic acid preservative solution of choice by mechanical disruption (e.

Transfer the supernatant to a clean tube. Extract the RNA with the method of choice following manufacturer instructions.

Avoid repeated freezing and thawing. The tubes must be handled with gloves at all times. After johnson war the samples always keep them on ice. Prepare DNAse-I mix by addition of (for 1 sample): 7.

If the take off condom are too diluted and the concentration is lower than expected, add larger volumes of take off condom RNA instead of water. Check your manufacturer instructions before performing RT-qPCR. NOTE: For relative quantification of mRNA according to the Ct method30 a reference gene must be selected for normalization of the Ct values.

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Comments:

09.05.2019 in 14:39 Викентий:
Хорошая работа!

12.05.2019 in 11:33 gartnarasi:
Я конечно, прошу прощения, но это мне совсем не подходит. Кто еще, может помочь?